Mouse Endothelin- 3, Edn3 ELISA KIT

Product Name:Mouse Endothelin- 3, Edn3 ELISA KIT
Packing:96T

Catalog No.:ELI-06803m

Gene Name:Mouse Edn3

Detect Range:0.156-10ng/ml

Sensitivity:0.094ng/ml

Target Protein Name:Mouse Edn3

Alternative Name:Mouse Endothelin-3, Edn3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Endothelin- 3, Edn3 ELISA KIT allows for the in vitro quantitative determination of Mouse Edn3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Endothelin- 3, Edn3 ELISA KIT has been pre-coated with an Mouse Endothelin-3, Edn3 antibody specific to Mouse Edn3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Edn3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Edn3 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Edn3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!