Mouse Endothelin B receptor, EDNRB ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY!

Synonyms

Mouse EdnrB ELISA KIT ,Mouse ABCDS ELISA KIT ,Mouse ET-B ELISA KIT ,Mouse ET-BR ELISA KIT ,Mouse ETB ELISA KIT ,Mouse ETB1 ELISA KIT ,Mouse ETBR ELISA KIT ,Mouse ETRB ELISA KIT ,Mouse HSCR ELISA KIT ,Mouse HSCR2 ELISA KIT ,Mouse WS4A ELISA KIT ,Mouse endothelin receptor non-selective type ELISA KIT ,Mouse endothelin receptor subtype B1 ELISA KIT ,Mouse endothelin receptor type  ELISA KIT ,Mouse Endothelin B receptor ELISA KIT

Intended use

Mouse EdnrB ELISA KIT allows for the in vitro quantitative determination of mouse endothelin b receptor concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided Mouse EdnrB ELISA KIT has been pre-coated with an antibody specific to ET-B. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for ET-B and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain ET-B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ET-B in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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