Mouse Eukaryotic translation initiation factor 5, Eif5 ELISA KIT

Product Name:Mouse Eukaryotic translation initiation factor 5, Eif5 ELISA KIT
Packing:96T

Catalog No.:ELI-20278m

Gene Name:Mouse Eif5

Detect Range:0.156-10ng/ml

Sensitivity:0.094ng/ml

Target Protein Name:Mouse Eif5

Alternative Name:Mouse Eukaryotic translation initiation factor 5, Eif5
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Eukaryotic translation initiation factor 5, Eif5 ELISA KIT allows for the in vitro quantitative determination of Mouse Eif5 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Eukaryotic translation initiation factor 5, Eif5 ELISA KIT has been pre-coated with an Mouse Eukaryotic translation initiation factor 5, Eif5 antibody specific to Mouse Eif5 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Eif5 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Eif5 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Eif5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!