Mouse Fatty acid 2- hydroxylase, Fa2h ELISA KIT
Product Name:Mouse Fatty acid 2- hydroxylase, Fa2h ELISA KIT
Packing:96T
Catalog No.:ELI-38515m
Gene Name:Mouse Fa2h
Detect Range:78.1-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Fa2h
Alternative Name:Mouse Fatty acid 2-hydroxylase, Fa2h
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Fatty acid 2- hydroxylase, Fa2h ELISA KIT allows for the in vitro quantitative determination of Mouse Fa2h concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Fatty acid 2- hydroxylase, Fa2h ELISA KIT has been pre-coated with an Mouse Fatty acid 2-hydroxylase, Fa2h antibody specific to Mouse Fa2h .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Fa2h and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Fa2h , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Fa2h in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!