Mouse FGFR3 ELISA KIT

Packing  96Tests

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

Gene Name  FGFR3

Protein Name  Fibroblast growth factor receptor 3

Alternative Name

Mouse FGFR3 ELISA KIT ,Mouse ACH ELISA KIT ,Mouse CD333 ELISA KIT ,Mouse CEK2 ELISA KIT ,Mouse HSFGFR3EX ELISA KIT ,Mouse JTK4 ELISA KIT ,Mouse fibroblast growth factor receptor 3 ELISA KIT ,Mouse FGFR-3 ELISA KIT ,Mouse fibroblast growth factor receptor 3 variant 4 ELISA KIT ,Mouse hydroxyaryl-protein kinase ELISA KIT ,Mouse tyrosine kinase JTK4 ELISA KIT

Intended use

Mouse FGFR3 ELISA KIT allows for the in vitro quantitative determination of FGFR3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Test principle      

The microtiter plate provided in Mouse FGFR3 ELISA KIT has been pre-coated with an FGFR3 antibody specific to FGFR3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for FGFR3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added  to  each  microplate  well  and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain FGFR3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of FGFR3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Sample collection and storage 

Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20