Mouse Fructose- 1,6- bisphosphatase 1, FBP1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

f16p1,FBP1,FBPase 1; FBP; fructose-bisphosphatase 1; growth-inhibiting protein 17; fructose-1,6-bisphosphatase 1, D-fructose-1,6-bisphosphate 1-phosphohydrolase 1

Search name

Mouse f16p1 ELISA KIT ,Mouse FBP1 ELISA KIT ,Mouse FBPase 1 ELISA KIT ,Mouse FBP ELISA KIT ,Mouse fructose-bisphosphatase 1 ELISA KIT ,Mouse growth-inhibiting protein 17 ELISA KIT ,Mouse fructose-1,6-bisphosphatase 1 ELISA KIT, Mouse D-fructose-1,6-bisphosphate 1-phosphohydrolase 1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of mouse fructose-1,6-bisphosphatase 1,FBPase 1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to FBPase 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for FBPase 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain FBPase 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of FBPase 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.