Mouse Gamma- crystallin F, Crygf ELISA KIT

Product Name:Mouse Gamma- crystallin F, Crygf ELISA KIT
Packing:96T

Catalog No.:ELI-31981m

Gene Name:Mouse Crygf

Detect Range:0.156-10ng/ml

Sensitivity:0.094ng/ml

Target Protein Name:Mouse Crygf

Alternative Name:Mouse Gamma-crystallin F, Crygf
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Gamma- crystallin F, Crygf ELISA KIT allows for the in vitro quantitative determination of Mouse Crygf concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Gamma- crystallin F, Crygf ELISA KIT has been pre-coated with an Mouse Gamma-crystallin F, Crygf antibody specific to Mouse Crygf .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Crygf and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Crygf , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Crygf in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!