Mouse Glutamate- cysteine ligase catalytic subunit, GCLC ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

GCLC,GCL; GCS; GLCL; GLCLC; GCS heavy chain; gamma-ECS; gamma-glutamylcysteine synthetase; glutamate--cysteine ligase catalytic subunit; glutamate-cysteine ligase, catalytic subunit,Glutamate--cysteine ligase catalytic subunit),Gamma-glutamylcysteine synthetase,GCS heavy chain,Gamma-ECS,

Search name

Mouse GCLC ELISA KIT ,Mouse GCL ELISA KIT ,Mouse GCS ELISA KIT ,Mouse GLCL ELISA KIT ,Mouse GLCLC ELISA KIT ,Mouse GCS heavy chain ELISA KIT ,Mouse gamma-ECS ELISA KIT ,Mouse gamma-glutamylcysteine synthetase ELISA KIT ,Mouse glutamate--cysteine ligase catalytic subunit ELISA KIT ,Mouse glutamate-cysteine ligase catalytic subunit ELISA KIT ,Mouse Glutamate--cysteine ligase catalytic subunit ELISA KIT ,Mouse Gamma-glutamylcysteine synthetase ELISA KIT ,Mouse GCS heavy chain ELISA KIT ,Mouse Gamma-ECS ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Mouse glutamate--cysteine ligase catalytic subunit,Glutamate--cysteine ligase catalytic subunit concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutamate--cysteine ligase catalytic subunit. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Glutamate--cysteine ligase catalytic subunit and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Glutamate--cysteine ligase catalytic subunit, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Glutamate--cysteine ligase catalytic subunit in the samples is then determined by comparing the O.D. of the samples to the standard curve.