Mouse GDP- L- fucose synthase, TSTA3 ELISA KIT

96 Tests

Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

TSTA3,Tstap35b,P35B; GDP-L-fucosesynthase, Protein FX,SDR4E1; 3-5 epimerase/4-reductase; GDP-4-keto-6-deoxy-D-mannose epimerase-reductase; GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase; red cell NADP(H)-binding protein; short chain dehydrogenase/reductase family 4E member 1; tissue specific transplantation antigen 3; tissue specific transplantation antigen P35B

Search name

Mouse TSTA3 ELISA KIT ,Mouse Tstap35b ELISA KIT ,Mouse P35B ELISA KIT ,Mouse GDP-L-fucosesynthase ELISA KIT ,Mouse  Protein FX ELISA KIT ,Mouse SDR4E1 ELISA KIT ,Mouse 3-5 epimerase/4-reductase ELISA KIT ,Mouse GDP-4-keto-6-deoxy-D-mannose epimerase-reductase ELISA KIT ,Mouse GDP-4-keto-6-deoxy-D-mannose-3 ELISA KIT ,Mouse 5-epimerase-4-reductase ELISA KIT ,Mouse red cell NADP(H)-binding protein ELISA KIT ,Mouse short chain dehydrogenase/reductase family 4E member 1 ELISA KIT ,Mouse tissue specific transplantation antigen 3 ELISA KIT ,Mouse tissue specific transplantation antigen P35B ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of mouseGDP-L-fucose synthaseconcentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

The ELISA is based on the competitive binding enzyme immunoassaytechnique. The microtiter plate provided in this kit has been pre-coated with an antibody specific toGDP-L-fucose synthase, During the reaction,GDP-L-fucose synthasein the sample or standard competes with a fixed amount of biotin-labeledGDP-L-fucose synthase for sites on a pre-coated Monoclonal antibody specific to GDP-L-fucose synthase. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Thena TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GDP-L-fucose synthase in the samples is then determined by comparing the O.D. of the samples to the standard curve.