Mouse Glutathione peroxidase 2,GPx2 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE

BEGINNING!

Synonyms

Glutathione peroxidase 2,GPx-2,GSHPx-2,Glutathione peroxidase-related protein 2,GPRP-2,Gastrointestinal glutathione peroxidase,Glutathione peroxidase-gastrointestinal,GPx-GI,GSHPx-GI, GPX2,GPx-2; GI-GPx; GPRP; GPRP-2; GPx-GI; GSHPX-GI; GSHPx-2; gastrointestinal glutathione peroxidase; glutathione peroxidase 2; glutathione peroxidase-related protein 2; glutathione peroxidase 2 (gastrointestinal)

Search name

Mouse Glutathione peroxidase 2 ELISA KIT ,Mouse GPx-2 ELISA KIT ,Mouse GSHPx-2 ELISA KIT ,Mouse Glutathione peroxidase-related protein 2 ELISA KIT ,Mouse GPRP-2 ELISA KIT ,Mouse Gastrointestinal glutathione peroxidase ELISA KIT ,Mouse Glutathione peroxidase-gastrointestinal ELISA KIT ,Mouse GPx-GI ELISA KIT ,Mouse GSHPx-GI ELISA KIT ,Mouse GPX2 ELISA KIT ,Mouse GPx-2 ELISA KIT ,Mouse GI-GPx ELISA KIT ,Mouse GPRP ELISA KIT ,Mouse GPRP-2 ELISA KIT ,Mouse GPx-GI ELISA KIT ,Mouse GSHPX-GI ELISA KIT ,Mouse GSHPx-2 ELISA KIT ,Mouse gastrointestinal glutathione peroxidase ELISA KIT ,Mouse glutathione peroxidase 2 ELISA KIT ,Mouse glutathione peroxidase-related protein 2 ELISA KIT ,Mouse glutathione peroxidase 2 (gastrointestinal) ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of mouse glutathione peroxidase 2,GPx-2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to GPx-2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GPx-2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GPx-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GPx-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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