Mouse H2-Ab1 ELISA KIT

Product Name:Mouse H2-Ab1 ELISA KIT
Packing:96T

Catalog No.:ELI-20429m

Gene Name:Mouse H2-Ab1

Detect Range:0.156-10ng/ml

Sensitivity:0.094ng/ml

Target Protein Name:Mouse H2-Ab1

Alternative Name:Mouse H2-Ab1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse H2-Ab1 ELISA KIT allows for the in vitro quantitative determination of Mouse H2-Ab1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse H2-Ab1 ELISA KIT has been pre-coated with an Mouse H2-Ab1 antibody specific to Mouse H2-Ab1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse H2-Ab1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse H2-Ab1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse H2-Ab1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!