Mouse Proheparin-binding EGF-like growth factor, HBEGF ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Proheparin-binding EGF-like growth factor, HBEGF,HB-EGF,DTR; DTS; DTSF; HEGFL; diphtheria toxin receptor (heparin-binding EGF-like growth factor); diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor); heparin-binding epidermal growth factor; proheparin-binding EGF-like growth factor; heparin-binding EGF-like growth factor

Search name

Mouse Proheparin-binding EGF-like growth factor ELISA KIT ,Mouse  HBEGF ELISA KIT ,Mouse HB-EGF ELISA KIT ,Mouse DTR ELISA KIT ,Mouse DTS ELISA KIT ,Mouse DTSF ELISA KIT ,Mouse HEGFL ELISA KIT ,Mouse diphtheria toxin receptor (heparin-binding EGF-like growth factor) ELISA KIT ,Mouse diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor) ELISA KIT ,Mouse heparin-binding epidermal growth factor ELISA KIT ,Mouse proheparin-binding EGF-like growth factor ELISA KIT ,Mouse heparin-binding EGF-like growth factor ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Mouse proheparin-binding egf-like growth factor,HBEGF concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to HBEGF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for HBEGF and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain HBEGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of HBEGF in the samples is then determined by comparing the O.D. of the samples to the standard curve.