Mouse Potassium/sodium hyperpolarization- activated cyclic nucleotide- gated channel 2, Hcn2 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

HCN2,BCNG-2; BCNG2; HAC-1; brain cyclic nucleotide gated channel 2; brain cyclic nucleotide-gated channel 2; potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2; hyperpolarization activated cyclic nucleotide-gated potassium channel 2,Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2,Brain cyclic nucleotide-gated channel 2,Hyperpolarization-activated cation channel 1,HAC-1

Search name

Mouse HCN2 ELISA KIT ,Mouse BCNG-2 ELISA KIT ,Mouse BCNG2 ELISA KIT ,Mouse HAC-1 ELISA KIT ,Mouse brain cyclic nucleotide gated channel 2 ELISA KIT ,Mouse brain cyclic nucleotide-gated channel 2 ELISA KIT ,Mouse potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 ELISA KIT ,Mouse hyperpolarization activated cyclic nucleotide-gated potassium channel 2 ELISA KIT ,Mouse Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 ELISA KIT ,Mouse Brain cyclic nucleotide-gated channel 2 ELISA KIT ,Mouse Hyperpolarization-activated cation channel 1 ELISA KIT ,Mouse HAC-1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of mouse potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2,HCN2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to HCN2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for HCN2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain HCN2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of HCN2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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