Mouse Helix- loop- helix protein 1, Nhlh1 ELISA KIT

Product Name:Mouse Helix- loop- helix protein 1, Nhlh1 ELISA KIT
Packing:96T

Catalog No.:ELI-12991m

Gene Name:Mouse Nhlh1

Detect Range:78.1-5000pg/ml

Sensitivity:46.9pg/ml

Target Protein Name:Mouse Nhlh1

Alternative Name:Mouse Helix-loop-helix protein 1, Nhlh1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Helix- loop- helix protein 1, Nhlh1 ELISA KIT allows for the in vitro quantitative determination of Mouse Nhlh1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Helix- loop- helix protein 1, Nhlh1 ELISA KIT has been pre-coated with an Mouse Helix-loop-helix protein 1, Nhlh1 antibody specific to Mouse Nhlh1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Nhlh1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Nhlh1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Nhlh1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!