Mouse Hematopoietic prostaglandin D synthase, HPGDS ELISA Kit

96 Tests

Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Hematopoietic prostaglandin D synthase,H-PGDS,GST, GST TAG,Prostaglandin-H2 D-isomerase,Glutathione-requiring prostaglandin D synthase,Glutathione-dependent PGD synthase,GST class-sigma,Glutathione S-transferase HpgdsH-PGDS; Ptgds2; GST class-sigma; glutathione S-transferase; glutathione-dependent PGD synthase; glutathione-dependent PGD synthetase; glutathione-requiring prostaglandin D synthase; prostaglandin D2 synthase 2, hematopoietic; prostaglandin-H2 D-isomerase; hematopoietic prostaglandin D synthase

Search name

Mouse Hematopoietic prostaglandin D synthase ELISA KIT ,Mouse H-PGDS ELISA KIT ,Mouse GST ELISA KIT ,Mouse  GST TAG ELISA KIT ,Mouse Prostaglandin-H2 D-isomerase ELISA KIT ,Mouse Glutathione-requiring prostaglandin D synthase ELISA KIT ,Mouse Glutathione-dependent PGD synthase ELISA KIT ,Mouse GST class-sigma ELISA KIT ,Mouse Glutathione S-transferase HpgdsH-PGDS ELISA KIT ,Mouse Ptgds2 ELISA KIT ,Mouse GST class-sigma ELISA KIT ,Mouse glutathione S-transferase ELISA KIT ,Mouse glutathione-dependent PGD synthase ELISA KIT ,Mouse glutathione-dependent PGD synthetase ELISA KIT ,Mouse glutathione-requiring prostaglandin D synthase ELISA KIT ,Mouse prostaglandin D2 synthase 2 ELISA KIT ,Mouse  hematopoietic ELISA KIT ,Mouse prostaglandin-H2 D-isomerase ELISA KIT ,Mouse hematopoietic prostaglandin D synthase ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Mouse hematopoietic prostaglandin d synthase,H-PGDS concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

The ELISA is based on the competitive binding enzyme immunoassay technique.The microtiter plate provided in this kit has been pre-coated with an antibody specific to H-PGDS, During the reaction, H-PGDS in the sample or standard competes with a fixed amount of biotin-labeled H-PGDS for sites on a pre-coated Monoclonal antibody specific to H-PGDS. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of H-PGDS in the samples is then determined by comparing the O.D. of the samples to the standard curve.