Mouse Hemoglobin subunit beta- H1, Hbb-bh1 ELISA KIT
Product Name:Mouse Hemoglobin subunit beta- H1, Hbb-bh1 ELISA KIT
Gene Name:Mouse Hbb-bh1
Target Protein Name:Mouse Hbb-bh1
Alternative Name:Mouse Hemoglobin subunit beta-H1, Hbb-bh1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Hemoglobin subunit beta- H1, Hbb-bh1 ELISA KIT allows for the in vitro quantitative determination of Mouse Hbb-bh1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Hemoglobin subunit beta- H1, Hbb-bh1 ELISA KIT has been pre-coated with an Mouse Hemoglobin subunit beta-H1, Hbb-bh1 antibody specific to Mouse Hbb-bh1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Hbb-bh1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Hbb-bh1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Hbb-bh1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!