Mouse Histone H3.2, Hist1h3b ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Hist1h3b,H3-53,H3.2,H3b,H3/l; H3FL; H3 histone family member L; histone 1 H3b; histone H3.1; histone H3/l; histone cluster 1 H3b
Search name
Mouse Hist1h3b ELISA KIT ,Mouse H3-53 ELISA KIT ,Mouse H3.2 ELISA KIT ,Mouse H3b ELISA KIT ,Mouse H3/l ELISA KIT ,Mouse H3FL ELISA KIT ,Mouse H3 histone family member L ELISA KIT ,Mouse histone 1 H3b ELISA KIT ,Mouse histone H3.1 ELISA KIT ,Mouse histone H3/l ELISA KIT ,Mouse histone cluster 1 H3b ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Histone H3.2, Hist1h3b concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hist1h3b. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Hist1h3b and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Hist1h3b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The