Mouse Histone H3.3, H3f3a ELISA KIT

Product Name:Mouse Histone H3.3, H3f3a ELISA KIT
Packing:96T

Catalog No.:ELI-08489m

Gene Name:Mouse H3f3a

Detect Range:31.25-2000pg/ml

Sensitivity:18.75pg/ml

Target Protein Name:Mouse H3f3a

Alternative Name:Mouse Histone H3.3, H3f3a
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Histone H3.3, H3f3a ELISA KIT allows for the in vitro quantitative determination of Mouse H3f3a concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Histone H3.3, H3f3a ELISA KIT has been pre-coated with an Mouse Histone H3.3, H3f3a antibody specific to Mouse H3f3a .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse H3f3a and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse H3f3a , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse H3f3a in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!