Mouse Homeobox protein orthopedia, Otp ELISA KIT

Product Name:Mouse Homeobox protein orthopedia, Otp ELISA KIT
Packing:96T

Catalog No.:ELI-44528m

Gene Name:Mouse Otp

Detect Range:15.6-1000pg/ml

Sensitivity:9.375pg/ml

Target Protein Name:Mouse Otp

Alternative Name:Mouse Homeobox protein orthopedia, Otp
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Homeobox protein orthopedia, Otp ELISA KIT allows for the in vitro quantitative determination of Mouse Otp concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Homeobox protein orthopedia, Otp ELISA KIT has been pre-coated with an Mouse Homeobox protein orthopedia, Otp antibody specific to Mouse Otp .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Otp and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Otp , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Otp in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!