Mouse Hormone- sensitive lipase, Lipe ELISA KIT

Product Name:Mouse Hormone- sensitive lipase, Lipe ELISA KIT
Packing:96T

Catalog No.:ELI-04222m

Gene Name:Mouse Lipe

Detect Range:78.125-5000pg/ml

Sensitivity:46.875pg/ml

Target Protein Name:Mouse Lipe

Alternative Name:Mouse Hormone-sensitive lipase, Lipe
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Hormone- sensitive lipase, Lipe ELISA KIT allows for the in vitro quantitative determination of Mouse Lipe concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Hormone- sensitive lipase, Lipe ELISA KIT has been pre-coated with an Mouse Hormone-sensitive lipase, Lipe antibody specific to Mouse Lipe .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Lipe and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Lipe , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Lipe in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!