Mouse Hsc70- interacting protein, St13 ELISA KIT
Product Name:Mouse Hsc70- interacting protein, St13 ELISA KIT
Gene Name:Mouse St13
Target Protein Name:Mouse St13
Alternative Name:Mouse Hsc70-interacting protein, St13
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Hsc70- interacting protein, St13 ELISA KIT allows for the in vitro quantitative determination of Mouse St13 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Hsc70- interacting protein, St13 ELISA KIT has been pre-coated with an Mouse Hsc70-interacting protein, St13 antibody specific to Mouse St13 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse St13 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse St13 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse St13 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!