Mouse 3 beta- hydroxysteroid dehydrogenase, HSD3B1 ELISA Kit

96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1,3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type
I(3-beta-HSD I),Trophoblast antigen FDO161G, HSD3B1,3BETAHSD; HSD3B; HSDB3; HSDB3A; SDR11E1; 3 beta-hydroxysteroid
dehydrogenase/Delta 5-->4-isomerase type 1; 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I; 3-beta-HSD I;
3-beta-hydroxy-5-ene steroid dehydrogenase; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; delta-5-3-ketosteroid isomerase;
hydroxy-delta-5-steroid dehydrogenase, 3 beta-and steroid delta-isomerase 1; progesterone reductase; short chain
dehydrogenase/reductase family 11E, member 1; steroid Delta-isomerase; trophoblast antigen FDO161G; hydroxy-delta-5-steroid
dehydrogenase, 3 beta- and steroid delta-isomerase 1

Search name
Mouse 3 beta-hydroxysteroid dehydrogenase ELISA Kit, Mouse Delta 5-->4-isomerase type 1 ELISA Kit, Mouse HSD3B1 ELISA
Kit, Mouse 3-beta-HSD I ELISA Kit, Mouse Delta 5-->4-isomerase type I ELISA Kit, Mouse Delta 5-->4-isomerase type 1 ELISA
Kit, Mouse Trophoblast antigen FDO161G ELISA Kit, Mouse 3BETAHSD ELISA Kit, Mouse HSD3B ELISA Kit, Mouse HSDB3
ELISA Kit, Mouse HSDB3A ELISA Kit, Mouse SDR11E1 ELISA Kit, Mouse 3-beta-hydroxy-5-ene steroid dehydrogenase ELISA
Kit, Mouse 3 beta-and steroid delta-isomerase 1 ELISA Kit, Mouse progesterone reductase ELISA Kit, Mouse short chain
dehydrogenase member 1 ELISA Kit, Mouse reductase family 11E member 1 ELISA Kit, Mouse steroid Delta-isomerase ELISA
Kit, Mouse trophoblast antigen FDO161G ELISA Kit, Mouse hydroxy-delta-5-steroid dehydrogenase ELISA Kit, Mouse 3 beta-
and steroid delta-isomerase 1 ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse 3 beta-hydroxysteroid dehydrogenase/delta
5-->4-isomerase type 1,HSD3B1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological
fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to HSD3B1. Standards or samples are then added
to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for HSD3B1 and Avidin
conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to
each well. Only those wells that contain HSD3B1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color.
The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of HSD3B1 in the samples is then determined by comparing
the O.D. of the samples to the standard curve.