Mouse Interferon alpha- 4, Ifna4 ELISA KIT
Product Name:Mouse Interferon alpha- 4, Ifna4 ELISA KIT
Packing:96T
Catalog No.:ELI-42256m
Gene Name:Mouse Ifna4
Detect Range:15.6-1000pg/ml
Target Protein Name:Mouse Ifna4
Alternative Name:Mouse Interferon alpha-4, Ifna4
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Interferon alpha- 4, Ifna4 ELISA KIT allows for the in vitro quantitative determination of Mouse Ifna4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Interferon alpha- 4, Ifna4 ELISA KIT has been pre-coated with an Mouse Interferon alpha-4, Ifna4 antibody specific to Mouse Ifna4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Ifna4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Ifna4 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Ifna4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!