Mouse Kallikrein 1 ELISA Kit
Product Name:Mouse Kallikrein 1 ELISA Kit
Packing:96T
Catalog No.:ELA-E0967m
Gene Name:Kallikrein1
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Mouse Kallikrein 1
Alternative Name:Kallikrein1,Mouse Kallikrein 1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Kallikrein 1 ELISA Kit allows for the in vitro quantitative determination ofMouse Kallikrein 1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Kallikrein 1 ELISA Kit has been pre-coated with an Kallikrein1 antibody specific to Mouse Kallikrein 1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Kallikrein 1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Kallikrein 1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Kallikrein 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!