Mouse Kininogen-1, KNG1 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Kininogen-1,Kininogen I,Thiol proteinase inhibitor, KNG1,kininogen-1; BDK; KNG; HMWK; alpha-2-thiol proteinase inhibitor; bradykinin; fitzgerald factor; high molecular weight kininogen; williams-Fitzgerald-Flaujeac factor; kininogen 1

Search name

Mouse Kininogen-1 ELISA KIT ,Mouse Kininogen I ELISA KIT ,Mouse Thiol proteinase inhibitor ELISA KIT ,Mouse KNG1 ELISA KIT ,Mouse kininogen-1 ELISA KIT ,Mouse BDK ELISA KIT ,Mouse KNG ELISA KIT ,Mouse HMWK ELISA KIT ,Mouse alpha-2-thiol proteinase inhibitor ELISA KIT ,Mouse bradykinin ELISA KIT ,Mouse fitzgerald factor ELISA KIT ,Mouse high molecular weight kininogen ELISA KIT ,Mouse williams-Fitzgerald-Flaujeac factor ELISA KIT ,Mouse kininogen 1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Mouse Kininogen-1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kininogen-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Kininogen-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Kininogen-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Kininogen-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.