Mouse Leptin receptor gene- related protein, Leprot ELISA KIT
Product Name:Mouse Leptin receptor gene- related protein, Leprot ELISA KIT
Packing:96T
Catalog No.:ELI-38221m
Gene Name:Mouse Leprot
Detect Range:78.1-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Leprot
Alternative Name:Mouse Leptin receptor gene-related protein, Leprot
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Leptin receptor gene- related protein, Leprot ELISA KIT allows for the in vitro quantitative determination of Mouse Leprot concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Leptin receptor gene- related protein, Leprot ELISA KIT has been pre-coated with an Mouse Leptin receptor gene-related protein, Leprot antibody specific to Mouse Leprot .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Leprot and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Leprot , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Leprot in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!