Mouse Leukocyte elastase inhibitor C, Serpinb1c ELISA KIT

Product Name:Mouse Leukocyte elastase inhibitor C, Serpinb1c ELISA KIT
Packing:96T

Catalog No.:ELI-42001m

Gene Name:Mouse Serpinb1c

Detect Range:78.1-5000pg/ml

Sensitivity:46.9pg/ml

Target Protein Name:Mouse Serpinb1c

Alternative Name:Mouse Leukocyte elastase inhibitor C, Serpinb1c
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Leukocyte elastase inhibitor C, Serpinb1c ELISA KIT allows for the in vitro quantitative determination of Mouse Serpinb1c concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Leukocyte elastase inhibitor C, Serpinb1c ELISA KIT has been pre-coated with an Mouse Leukocyte elastase inhibitor C, Serpinb1c antibody specific to Mouse Serpinb1c .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Serpinb1c and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Serpinb1c , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Serpinb1c in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!