Mouse Lysine- specific demethylase 4D, Kdm4d ELISA KIT
Product Name:Mouse Lysine- specific demethylase 4D, Kdm4d ELISA KIT
Packing:96T
Catalog No.:ELI-23735m
Gene Name:Mouse Kdm4d
Detect Range:31.25-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Kdm4d
Alternative Name:Mouse Lysine-specific demethylase 4D, Kdm4d
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Lysine- specific demethylase 4D, Kdm4d ELISA KIT allows for the in vitro quantitative determination of Mouse Kdm4d concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Lysine- specific demethylase 4D, Kdm4d ELISA KIT has been pre-coated with an Mouse Lysine-specific demethylase 4D, Kdm4d antibody specific to Mouse Kdm4d .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Kdm4d and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Kdm4d , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Kdm4d in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!