Mouse Mast cell protease 1, MCT1 ELISA Kit

Mouse Mast cell protease 1, MCT1 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Chymase,Mast cell protease I,Mast cell protease 5(mMCP-5),Alpha-chymase,Mast cell chymase 1, CMA1,Chymase,chymase 1, mast cell; chymase, mast cell; chymase, heart; chymase 1 preproprotein transcript I; chymase 1 preproprotein transcript E; alpha-chymase; chymase; CYH; MCT1; mast cell protease I

Search name

Mouse CMA1 ELISA KIT ,Mouse Chymase ELISA KIT ,Mouse chymase 1 ELISA KIT ,Mouse  mast cell ELISA KIT ,Mouse chymase mast cell ELISA KIT ,Mouse chymase heart ELISA KIT ,Mouse chymase 1 preproprotein transcript I ELISA KIT ,Mouse chymase 1 preproprotein transcript E ELISA KIT ,Mouse alpha-chymase ELISA KIT ,Mouse chymase ELISA KIT ,Mouse CYH ELISA KIT ,Mouse MCT1 ELISA KIT ,Mouse mast cell protease I ELISA KIT, Mouse CYH ELISA KIT, Mouse CYM ELISA KIT, Mouse Alpha-chymase ELISA KIT, Mouse MCPT5 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of mouse Mast cell protease I concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mast cell protease I. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Mast cell protease I and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mast cell protease I, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mast cell protease I in the samples is then determined by comparing the O.D. of the samples to the standard curve.