Mouse Matrix metalloproteinase- 25, Mmp25 ELISA KIT

Product Name:Mouse Matrix metalloproteinase- 25, Mmp25 ELISA KIT
Packing:96T

Catalog No.:ELI-46310m

Gene Name:Mouse Mmp25

Detect Range:0.156-10ng/ml

Sensitivity:0.094ng/ml

Target Protein Name:Mouse Mmp25

Alternative Name:Mouse Matrix metalloproteinase-25, Mmp25
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Matrix metalloproteinase- 25, Mmp25 ELISA KIT allows for the in vitro quantitative determination of Mouse Mmp25 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Matrix metalloproteinase- 25, Mmp25 ELISA KIT has been pre-coated with an Mouse Matrix metalloproteinase-25, Mmp25 antibody specific to Mouse Mmp25 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Mmp25 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Mmp25 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Mmp25 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!