Mouse Multiple coagulation factor deficiency protein 2, MCFD2 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Multiple coagulation factor deficiency protein 2,Neural stem cell-derived neuronal survival protein,MCFD2,F5F8D; F5F8D2; LMAN1IP; SDNSF; multiple coagulation factor deficiency protein 2; neural stem cell-derived neuronal survival protein; multiple coagulation factor deficiency 2

Search name

Mouse Multiple coagulation factor deficiency protein 2 ELISA KIT ,Mouse Neural stem cell-derived neuronal survival protein ELISA KIT ,Mouse MCFD2 ELISA KIT ,Mouse F5F8D ELISA KIT ,Mouse F5F8D2 ELISA KIT ,Mouse LMAN1IP ELISA KIT ,Mouse SDNSF ELISA KIT ,Mouse multiple coagulation factor deficiency protein 2 ELISA KIT ,Mouse neural stem cell-derived neuronal survival protein ELISA KIT ,Mouse multiple coagulation factor deficiency 2 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Mouse multiple coagulation factor deficiency protein 2,MCFD2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to MCFD2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for MCFD2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain MCFD2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of MCFD2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.