Mouse Mesoderm- specific transcript protein, Mest ELISA KIT

Product Name:Mouse Mesoderm- specific transcript protein, Mest ELISA KIT
Packing:96T

Catalog No.:ELI-38977m

Gene Name:Mouse Mest

Detect Range:0.15-10ng/ml

Sensitivity:0.094ng/ml

Target Protein Name:Mouse Mest

Alternative Name:Mouse Mesoderm-specific transcript protein, Mest
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Mesoderm- specific transcript protein, Mest ELISA KIT allows for the in vitro quantitative determination of Mouse Mest concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Mesoderm- specific transcript protein, Mest ELISA KIT has been pre-coated with an Mouse Mesoderm-specific transcript protein, Mest antibody specific to Mouse Mest .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Mest and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Mest , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Mest in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!