Mouse MFN2 ELISA KIT
Packing 96Tests Manual
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Gene Name MFN2
Protein Name Mitofusin-2
Alternative Name
Mouse MFN2 ELISA KIT , Mouse transmembrane GTPase MFN2 ELISA KIT ,Mouse CMT2A ELISA KIT ,Mouse CMT2A2 ELISA KIT ,Mouse CMT2A2A ELISA KIT ,Mouse CMT2A2B ELISA KIT ,Mouse CPRP1 ELISA KIT ,Mouse HMSN6A ELISA KIT ,Mouse HSG ELISA KIT ,Mouse MARF ELISA KIT ,Mouse mitofusin-2 ELISA KIT ,Mouse hyperplasia suppressor ELISA KIT ,Mouse mitochondrial assembly regulatory factor ELISA KIT ,Mouse mitofusin 2 ELISA KIT
REAGENTS AND MATERIALS PROVIDED】
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Reagents
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Quantity
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Reagents
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Quantity
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Assay plate (96 Wells)
ELI-31284M-1
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1
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CoA
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1
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Standard (lyophilized)
ELI-31284M--2
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2
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Sample Diluent
ELI-31284M-7
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1 x 20 mL
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Biotin-Conjugate (concentrate 100 x)
ELI-31284M-3
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1 x 120μL
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Biotin-Conjugate Diluent
ELI-31284M-8
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1 x 20 mL
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Streptavidin-HRP (concentrate 100 x)
ELI-31284M-4
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1 x 120μL
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Streptavidin-HRP Diluent
ELI-31284M-9
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1 x 20 mL
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Wash Buffer (concentrate 25 x)
ELI-31284M-5
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1 x 20 mL
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Substrate Solution
ELI-31284M-10
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1 x 12 mL
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Stop Solution
ELI-31284M-6
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1 x 10 mL
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Adhesive Films
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3
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【MATERIALS REQUIRED BUT NOT SUPPLIED】
※ Microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
※ Precision single or multi-channel pipettes and disposable tips.
※ Deionized or distilled water.
※ Eppendorf Tubes for serial dilution samples.
※ Container for Wash Solution.
※ Absorbent paper for blotting the microtiter plate.
【STORAGE】
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Unopened kit
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Store at 2 - 8°C. Do not use past kit in expiration date.
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Opened/ Reconstituted Reagents
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Coated assay plate
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May be stored for up to 1 month at 2 - 8°C. Return unused wells to the foil pouch containing the desiccant pack, reseal along entire edge of zip-seal, and avoid the damp.
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Standard
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May be stored for up to 1 month at 2 - 8°C. If not for recent use, better keep it store at -20°C.
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Biotin-Conjugate
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Streptavidin-HRP
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Sample Diluent
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May be stored for up to 1 month at 2 - 8°C.
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Biotin-Conjugate
Diluent
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Streptavidin-HRP
Diluent
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Wash Buffer
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Substrate Solution
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Stop Solution
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【INTRODUCTION】
Mitofusin-2 is a mitochondrial membrane protein that participates in mitochondrial fusion and contributes to the maintenance and operation of the mitochondrial network. This protein is involved in the regulation of vascular smooth muscle cell 4 proliferation, and it may play a role in the pathophysiology of obesity. Mutations in this gene cause Charcot-Marie-Tooth disease type 2A2, and hereditary motor and sensory neuropathy VI, which are both disorders of the peripheral nervous system. Defects in this gene have also been associated with early-onset
【PRINCIPLE OF THE ASSAY】
This assay employs a two-site sandwich ELISA to quantitate MFN2 in Mouse serum, plasma. An antibody specific for MFN2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MFN2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MFN2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MFN2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Caution:
1. This product is FOR RESEARCH USE ONLY!
English