Mouse Myosin light chain kinase, smooth muscle, Mylk ELISA KIT

Product Name:Mouse Myosin light chain kinase, smooth muscle, Mylk ELISA KIT
Packing:96T

Catalog No.:ELI-03626m

Gene Name:Mouse Mylk

Detect Range:15.6-1000pg/ml

Sensitivity:9.375pg/ml

Target Protein Name:Mouse Mylk

Alternative Name:Mouse Myosin light chain kinase, smooth muscle, Mylk
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Myosin light chain kinase, smooth muscle, Mylk ELISA KIT allows for the in vitro quantitative determination of Mouse Mylk concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Myosin light chain kinase, smooth muscle, Mylk ELISA KIT has been pre-coated with an Mouse Myosin light chain kinase, smooth muscle, Mylk antibody specific to Mouse Mylk .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Mylk and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Mylk , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Mylk in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!