Mouse Myosin- Va, Myo5a ELISA KIT

Product Name:Mouse Myosin- Va, Myo5a ELISA KIT
Packing:96T

Catalog No.:ELI-13894m

Gene Name:Mouse Myo5a

Detect Range:78-5000pg/ml

Sensitivity:46.9pg/ml

Target Protein Name:Mouse Myo5a

Alternative Name:Mouse Myosin-Va, Myo5a
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Myosin- Va, Myo5a ELISA KIT allows for the in vitro quantitative determination of Mouse Myo5a concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Myosin- Va, Myo5a ELISA KIT has been pre-coated with an Mouse Myosin-Va, Myo5a antibody specific to Mouse Myo5a .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Myo5a and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Myo5a , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Myo5a in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!