Mouse Neprilysin, MME ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

CD10,neprilysin; CALLA; NEP; SFE; atriopeptidase; common acute lymphocytic leukemia antigen; enkephalinase; membrane metallo-endopeptidase (neutral endopeptidase, enkephalinase); membrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10); membrane metallo-endopeptidase variant 1; membrane metallo-endopeptidase variant 2; neutral endopeptidase 24.11; skin fibroblast elastase; membrane metallo-endopeptidase,Neprilysin,Skin fibroblast elastase,SFE,Neutral endopeptidase 24.11,NEP,Neutral endopeptidase,Enkephalinase,Atriopeptidase,Common acute lymphocytic leukemia antigen,CALLA,

Search name

Mouse CD10 ELISA KIT ,Mouse neprilysin ELISA KIT ,Mouse CALLA ELISA KIT ,Mouse NEP ELISA KIT ,Mouse SFE ELISA KIT ,Mouse atriopeptidase ELISA KIT ,Mouse common acute lymphocytic leukemia antigen ELISA KIT ,Mouse enkephalinase ELISA KIT ,Mouse membrane metallo endopeptidase ELISA KIT ,Mouse neutral endopeptidase enkephalinase ELISA KIT ,Mouse membrane metallo-endopeptidase ELISA KIT ,Mouse neutral endopeptidase enkephalinase ELISA KIT ,Mouse  CALLA ELISA KIT ,Mouse  CD10 ELISA KIT ,Mouse membrane metallo-endopeptidase variant 1 ELISA KIT ,Mouse membrane metallo-endopeptidase variant 2 ELISA KIT ,Mouse neutral endopeptidase 24.11 ELISA KIT ,Mouse skin fibroblast elastase ELISA KIT ,Mouse membrane metallo-endopeptidase ELISA KIT ,Mouse Neprilysin ELISA KIT ,Mouse Skin fibroblast elastase ELISA KIT ,Mouse SFE ELISA KIT ,Mouse Neutral endopeptidase 24.11 ELISA KIT ,Mouse NEP ELISA KIT ,Mouse Neutral endopeptidase ELISA KIT ,Mouse Enkephalinase ELISA KIT ,Mouse Atriopeptidase ELISA KIT ,Mouse Common acute lymphocytic leukemia antigen ELISA KIT ,Mouse CALLA ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of mouse Neprilysin concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neprilysin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Neprilysin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Neprilysin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Neprilysin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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