Mouse Nesfatin,NEFA ELISA Kit

96 Tests

Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Nesfatin-1, NEFA,nesfatin-1; HEL-S-109; DNA-binding protein NEFA; epididymis secretory protein Li 109; gastric cancer antigen Zg4; nucleobindin-2; nucleobinding 2; prepronefastin; prepronesfatin; nucleobindin 2

Search name

Mouse NEFA ELISA KIT ,Mouse Nesfatin-1 ELISA KIT ,Mouse nesfatin-1 ELISA KIT ,Mouse HEL-S-109 ELISA KIT ,Mouse DNA-binding protein NEFA ELISA KIT ,Mouse epididymis secretory protein Li 109 ELISA KIT ,Mouse gastric cancer antigen Zg4 ELISA KIT ,Mouse nucleobindin-2 ELISA KIT ,Mouse nucleobinding 2 ELISA KIT ,Mouse prepronefastin ELISA KIT ,Mouse prepronesfatin ELISA KIT ,Mouse nucleobindin 2 ELISA KIT

Intended use

Mouse NEFA ELISA KIT allows for the in vitro quantitative determination of Mouse Nesfatin-1 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

Mouse NEFA ELISA KIT is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nesfatin-1, During the reaction, Nesfatin-1 in the sample or standard competes with a fixed amount of biotin-labeled Nesfatin-1 for sites on a pre-coated Monoclonal antibody specific to Nesfatin-1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Nesfatin-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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