Mouse Neural cell adhesion molecule L1, L1CAM ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Neural cell adhesion molecule L1,N-CAM-L1,NCAM-L1, L1CAM,NCAM-L1; CAML1; CD171; HSAS; HSAS1; MASA; MIC5; N-CAM-L1; N-CAML1; S10; SPG1; antigen identified by monoclonal antibody R1; neural cell adhesion molecule L1; L1 cell adhesion molecule

Search name

Mouse Neural cell adhesion molecule L1 ELISA KIT ,Mouse N-CAM-L1 ELISA KIT ,Mouse NCAM-L1 ELISA KIT ,Mouse  L1CAM ELISA KIT ,Mouse NCAM-L1 ELISA KIT ,Mouse CAML1 ELISA KIT ,Mouse CD171 ELISA KIT ,Mouse HSAS ELISA KIT ,Mouse HSAS1 ELISA KIT ,Mouse MASA ELISA KIT ,Mouse MIC5 ELISA KIT ,Mouse N-CAM-L1 ELISA KIT ,Mouse N-CAML1 ELISA KIT ,Mouse S10 ELISA KIT ,Mouse SPG1 ELISA KIT ,Mouse antigen identified by monoclonal antibody R1 ELISA KIT ,Mouse neural cell adhesion molecule L1 ELISA KIT ,Mouse L1 cell adhesion molecule ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Mouse neural cell adhesion molecule l1,N-CAM-L1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-CAM-L1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for N-CAM-L1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain N-CAM-L1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of N-CAM-L1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.