Mouse Nodal, Nodal ELISA KIT

Product Name:Mouse Nodal, Nodal ELISA KIT
Packing:96T

Catalog No.:ELI-23583m

Gene Name:Mouse Nodal

Detect Range:78-5000pg/ml

Sensitivity:46.9pg/ml

Target Protein Name:Mouse Nodal

Alternative Name:Mouse Nodal, Nodal
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Nodal, Nodal ELISA KIT allows for the in vitro quantitative determination of Mouse Nodal concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Nodal, Nodal ELISA KIT has been pre-coated with an Mouse Nodal, Nodal antibody specific to Mouse Nodal .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Nodal and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Nodal , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Nodal in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!