Mouse Patched domain- containing protein 3, Ptchd3 ELISA KIT

Product Name:Mouse Patched domain- containing protein 3, Ptchd3 ELISA KIT
Packing:96T

Catalog No.:ELI-15541m

Gene Name:Mouse Ptchd3

Detect Range:0.15-10ng/ml

Sensitivity:0.094ng/ml

Target Protein Name:Mouse Ptchd3

Alternative Name:Mouse Patched domain-containing protein 3, Ptchd3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Patched domain- containing protein 3, Ptchd3 ELISA KIT allows for the in vitro quantitative determination of Mouse Ptchd3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Patched domain- containing protein 3, Ptchd3 ELISA KIT has been pre-coated with an Mouse Patched domain-containing protein 3, Ptchd3 antibody specific to Mouse Ptchd3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Ptchd3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Ptchd3 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Ptchd3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!