Mouse Pyruvate dehydrogenase E1 component subunit alpha, testis- specific form, mitochondrial, PDHA2 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial,; PDHE1-A type II,PDHA2,PDHAL; PDHE1-A type II; pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial; pyruvate dehydrogenase, E1-alpha polypeptide, testis specific; pyruvate dehydrogenase (lipoamide) alpha 2

Search name

Mouse Pyruvate dehydrogenase E1 component subunit alpha ELISA KIT ,Mouse  testis-specific form ELISA KIT ,Mouse  mitochondrial ELISA KIT ,Mouse  ELISA KIT ,Mouse PDHE1-A type II ELISA KIT ,Mouse PDHA2 ELISA KIT ,Mouse PDHAL ELISA KIT ,Mouse PDHE1-A type II ELISA KIT ,Mouse pyruvate dehydrogenase E1 component subunit alpha ELISA KIT ,Mouse  testis-specific form ELISA KIT ,Mouse  mitochondrial ELISA KIT ,Mouse pyruvate dehydrogenase ELISA KIT ,Mouse  E1-alpha polypeptide ELISA KIT ,Mouse  testis specific ELISA KIT ,Mouse pyruvate dehydrogenase (lipoamide) alpha 2 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Mouse pyruvate dehydrogenase e1 component subunit alpha, testis-specific form, mitochondrial,PDHE1-A type II concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to PDHE1-A type II. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PDHE1-A type II and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PDHE1-A type II, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PDHE1-A type II in the samples is then determined by comparing the O.D. of the samples to the standard curve.