Mouse Perilipin- 5, Plin5 ELISA KIT
Product Name:Mouse Perilipin- 5, Plin5 ELISA KIT
Packing:96T
Catalog No.:ELI-37335m
Gene Name:Mouse Plin5
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Plin5
Alternative Name:Mouse Perilipin-5, Plin5
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Perilipin- 5, Plin5 ELISA KIT allows for the in vitro quantitative determination of Mouse Plin5 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Perilipin- 5, Plin5 ELISA KIT has been pre-coated with an Mouse Perilipin-5, Plin5 antibody specific to Mouse Plin5 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Plin5 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Plin5 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Plin5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!