Mouse PFKFB4 ELISA KIT
Packing 96Tests
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Gene Name PFKFB4
Protein Name leukotriene A4 hydrolase
Alternative Name
Mouse PFKFB4 ELISA KIT ;Mouse 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 ELISA KIT ;Mouse 6PF-2-K/Fru-2,6-P2ase 4 ELISA KIT ;Mouse 6PF-2-K/Fru-2,6-P2ase testis-type isozyme ELISA KIT ;Mouse PFK/FBPase 4 ELISA KIT ;Mouse bifunctional enzyme with kinase and biphosphatase activities ELISA KIT ;Mouse 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 ELISA KIT ;
Intended use
Mouse PFKFB4 ELISA KIT allows for the in vitro quantitative determination of PFKFB4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Reagent | Quantity |
Assay plate | 1 |
Standard | 2 |
Sample Diluent | 1 × 20mL |
Assay Diluent A | 1 × 10mL |
Assay Diluent B | 1 × 10mL |
Detection Reagent A | 1 × 120μL |
Detection Reagent B | 1 × 120μL |
Wash Buffer(25 x concentrate) | 1 × 30mL |
Substrate | 1 × 10mL |
Stop Solution | 1 × 10mL |
Plate sealer | 5 |
Test principle
The microtiter plate provided in Mouse PFKFB4 ELISA KIT has been pre-coated with an PFKFB4 antibody specific to PFKFB4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for PFKFB4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PFKFB4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PFKFB4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample collection and storage
Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20