Mouse Polyglutamine- binding protein 1, PQBP1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

PQBP1,MRX2; MRX55; MRXS3; MRXS8; NPW38; RENS1; SHS; 38 kDa nuclear protein containing a WW domain; mental retardation, X-linked 2 (non-dysmorphic); nuclear protein containing WW domain 38 kD; polyglutamine tract-binding protein 1; polyglutamine-binding protein 1; polyglutamine binding protein 1

Search name

Mouse PQBP1 ELISA KIT ,Mouse MRX2 ELISA KIT ,Mouse MRX55 ELISA KIT ,Mouse MRXS3 ELISA KIT ,Mouse MRXS8 ELISA KIT ,Mouse NPW38 ELISA KIT ,Mouse RENS1 ELISA KIT ,Mouse SHS ELISA KIT ,Mouse 38 kDa nuclear protein containing a WW domain ELISA KIT ,Mouse mental retardation X-linked 2 ELISA KIT ,Mouse nuclear protein containing WW domain 38 kD ELISA KIT ,Mouse polyglutamine tract-binding protein 1 ELISA KIT ,Mouse polyglutamine-binding protein 1 ELISA KIT ,Mouse polyglutamine binding protein 1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Mouse Polyglutamine- binding protein 1, PQBP1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Polyglutamine- binding protein 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Polyglutamine- binding protein 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Polyglutamine- binding protein 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Polyglutamine- binding protein 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.