Mouse Protein disulfide- isomerase A4, Pdia4 ELISA KIT
Product Name:Mouse Protein disulfide- isomerase A4, Pdia4 ELISA KIT
Packing:96T
Catalog No.:ELI-15266m
Gene Name:Mouse Pdia4
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Pdia4
Alternative Name:Mouse Protein disulfide-isomerase A4, Pdia4
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Protein disulfide- isomerase A4, Pdia4 ELISA KIT allows for the in vitro quantitative determination of Mouse Pdia4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Protein disulfide- isomerase A4, Pdia4 ELISA KIT has been pre-coated with an Mouse Protein disulfide-isomerase A4, Pdia4 antibody specific to Mouse Pdia4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Pdia4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Pdia4 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Pdia4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!