Mouse Protein disulfide- isomerase, P4hb ELISA KIT

Product Name:Mouse Protein disulfide- isomerase, P4hb ELISA KIT
Packing:96T

Catalog No.:ELI-03451m

Gene Name:Mouse P4hb

Detect Range:31.25-2000pg/ml

Sensitivity:18.75pg/ml

Target Protein Name:Mouse P4hb

Alternative Name:Mouse Protein disulfide-isomerase, P4hb
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Protein disulfide- isomerase, P4hb ELISA KIT allows for the in vitro quantitative determination of Mouse P4hb concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Protein disulfide- isomerase, P4hb ELISA KIT has been pre-coated with an Mouse Protein disulfide-isomerase, P4hb antibody specific to Mouse P4hb .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse P4hb and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse P4hb , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse P4hb in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!