Mouse Protein flightless- 1 homolog, Flii ELISA KIT
Product Name:Mouse Protein flightless- 1 homolog, Flii ELISA KIT
Gene Name:Mouse Flii
Target Protein Name:Mouse Flii
Alternative Name:Mouse Protein flightless-1 homolog, Flii
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Protein flightless- 1 homolog, Flii ELISA KIT allows for the in vitro quantitative determination of Mouse Flii concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Protein flightless- 1 homolog, Flii ELISA KIT has been pre-coated with an Mouse Protein flightless-1 homolog, Flii antibody specific to Mouse Flii .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Flii and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Flii , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Flii in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!