Mouse Protein Wnt- 5b, Wnt5b ELISA KIT

Product Name:Mouse Protein Wnt- 5b, Wnt5b ELISA KIT
Packing:96T

Catalog No.:ELI-05129m

Gene Name:Mouse Wnt5b

Detect Range:0.15-10ng/ml

Sensitivity:0.094ng/ml

Target Protein Name:Mouse Wnt5b

Alternative Name:Mouse Protein Wnt-5b, Wnt5b
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Protein Wnt- 5b, Wnt5b ELISA KIT allows for the in vitro quantitative determination of Mouse Wnt5b concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Protein Wnt- 5b, Wnt5b ELISA KIT has been pre-coated with an Mouse Protein Wnt-5b, Wnt5b antibody specific to Mouse Wnt5b .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Wnt5b and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Wnt5b , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Wnt5b in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!