Mouse PTP synthase, PTPS ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

PTPS,PTS,PTP synthase; 6-pyruvoyltetrahydropterin synthase

Search name

Mouse PTPS ELISA KIT ,Mouse PTS ELISA KIT ,Mouse PTP synthase ELISA KIT ,Mouse 6-pyruvoyltetrahydropterin synthase ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Mouse PTP synthase, PTPS concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PTP synthase,PTPS. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Mouse PTP synthase,PTPS and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse PTP synthase,PTPS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse PTP synthase,PTPS in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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