Mouse Rab5 GDP/GTP exchange factor, RABGEF1 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

RABGEF1,RABEX5; RAP1; rabex-5; rabaptin-5-associated exchange factor for Rab5; RAB guanine nucleotide exchange factor (GEF) 1

Search name

Mouse RABGEF1 ELISA KIT ,Mouse RABEX5 ELISA KIT ,Mouse RAP1 ELISA KIT ,Mouse rabex-5 ELISA KIT ,Mouse rabaptin-5-associated exchange factor for Rab5 ELISA KIT ,Mouse RAB guanine nucleotide exchange factor (GEF) 1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Mouse Rab5 GDP/GTP exchange factor, RABGEF1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to RABGEF1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for RABGEF1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain RABGEF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of RABGEF1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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